Inceptum Chromatography System

Inceptum™ is a fully automated personal chromatography system for purification of proteins, RNA, and DNA. The system utilized application specific and open cartridges to ensure that the fluid path is completely separated from the instrument. The Inceptum™ system consists of an instrument and two types of disposable cartridges, and a Windows™ computer (not included). Application-specific cartridges contain up to two columns and include all the necessary buffer solutions to perform purification of proteins using specific chromatography. The application-specific cartridges can be utilized for the following types of purification:

• Affinity purification of antibodies using protein G binding, followed by buffer exchange into a neutral buffer, e.g. PBS.
• Ni-NTA affinity purification of His-tagged proteins.
• Buffer exchange cartridges to replace buffer using desalting columns. The standard buffer is PBS, but this can be customized for each order.
• Affinity purification of GST-tagged proteins.
• Affinity purification of MBP-tagged proteins.
• Ion-exchange cartridges that contain an IEX column and buffers for NaCl gradient elution.
• Custom cartridges with custom configurations of up to two columns and up to three buffer solutions.

The open cartridges provide all the necessary external connections to attach up to two columns and up to three buffer solutions, as well as a sample inlet. The sizes of the columns and volumes of buffer solutions are limited only by the chosen application protocol. Inceptum™ provides a disposable, easy to change, fluid drive and measurement system for user-defined purification.